FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5382545
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC541531.0060854112692044No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA384530.7144018303609166No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG376570.6996132870231461No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA231700.4304655139901292No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT216480.40218892735685446No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG206020.38275574101099014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197080.3661464976140469No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC186500.3464903684037941No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC171030.31774931746970997No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT150760.28009055196008575No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC138620.2575361655127825No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC137990.2563657154747429No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC137130.25476795827995863No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA120500.22387179299011897No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG107870.20040705651322935No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA107590.19988685649632285No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT97920.18192137734101618No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG92180.17125727699443294No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG83080.15435077644497167No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA82840.15390489071619465No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA82270.15284591211034929No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT80930.15035638345801103No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG78000.14491286185252514No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG77100.1432407903696114No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA73970.13742569732347804No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC73920.13733280446331614No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG70620.1312018756926324No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA66450.12345461115513201No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT62470.11606033948624675No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA58580.10883327496565287No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC56050.10413289624146198No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC54050.10041718183498698No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT54010.10034286754685748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG171650.069.460614
GTAGGTT272600.069.35978
TTGGCGA154950.069.2905417
AGTAGGT273550.069.272347
GTAGTAG521950.069.2598655
TAGGTTG272950.069.257949
GAGGTAG582250.069.197312
CGTAAAT172500.069.177279
ATTGGCG167900.069.1563216
GTTGTAT287050.069.0994312
GTTGACA209500.069.07323518
AGCTTAT339850.069.063932
GGTAGTA525500.069.0583654
TGAGGTA568950.069.045141
TGTATAG369100.069.04127514
AGGTTGT328400.068.9977210
TATAGTT327700.068.9648516
GCTTATC340250.068.9621663
TAGTAGG274450.068.944456
TTTGTGC146900.068.9412112
Produced by FastQC (version 0.11.5)