FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6110431
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA575820.942355784722878No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC503630.8242135456565993No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG451500.7389004147170634No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA309360.5062817991071333No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT277470.4540923545327653No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG247130.40443955590039393No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC237750.3890887565868922No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA225820.3695647655623638No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT207820.34010694171982303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197710.3235614639949293No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC197000.32239951649891796No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC193520.3167043372226935No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC169860.2779836643274427No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC169140.27680535137374107No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA157790.2582305568952501No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG151350.24769120214269666No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC132590.2169896035156931No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT128080.2096087820973676No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA124770.20419181560187818No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA123900.20276802078282202No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG119950.19630366499515337No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC112290.18376772440438327No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG111490.18245848778915924No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC109600.17936541628569247No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA104780.17147726567896765No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG98230.16075789089182088No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG90300.14778008294341266No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT84620.13848450297532203No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT82730.13539143147185526No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT81990.13418038760277304No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA81430.1332639219721162No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG78340.12820699554581338No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA78160.12791241730738798No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA77850.12740508811898865No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT77320.12653771886140275No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA74060.12120257965436482No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC71370.116800271535674No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC70660.11563832403966266No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAACTGTAGGCACCAT69010.11293802352076311No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT68040.11135057412480397No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGAACTGTAGGCACCATC67900.11112145771713976No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAACTGTAGGCA65110.10655549502154595No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA63880.104542543725639No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA63100.10326603802579556No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT61260.1002547938107803No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA61200.10015660106463847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT279500.069.423298
AGTAGGT279950.069.3616947
CAGCACG187850.069.355034
TTGGCGA170600.069.3320117
GTTGACA285650.069.3291318
AGCTTAT473000.069.168332
GTTGTAT293800.069.16598512
TGTATAG385750.069.1526714
ATTGGCG184650.069.11606616
GAGGTAG621000.069.099182
GCTTATC473000.069.078973
GCAGGTA50800.069.0234316
TAGTAGG281600.069.0191046
TAGGTTG280950.069.015179
GGTAGTA562400.069.0115054
GTAGTAG558250.069.004245
TGCAGGT50900.068.9565815
CAGTGGC129300.068.905835
TATAGTT342550.068.9049716
TAGCAGC204900.068.904791
Produced by FastQC (version 0.11.5)