FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5315927
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA536841.0098709030428747No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC443930.8350942366213832No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG392890.7390808790263673No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA330660.622017570971159No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC272420.5124600093266894No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT261490.49189915512383825No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG245900.46257219107786846No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC214220.4029776932602724No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT211010.39693923562155764No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA191000.3592976352007844No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA182420.34315745870851877No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC167890.31582450248094074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167530.3151472922784681No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC161690.30416143788280015No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG154530.290692479411399No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC148270.2789165464461796No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT142090.2672911046370652No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA116400.21896463213283401No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG115580.2174220977827574No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC110640.2081292688932711No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA107860.20289970121862094No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA106950.2011878643179261No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC102930.19362568372364783No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG102070.19200790379551863No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG93740.17633801216608128No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC88890.16721448582721318No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA87200.16403536015449421No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG83440.1569622758175573No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT82940.1560217060919008No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC81460.15323761970395755No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC77630.14603285560542872No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA76380.1436814312912875No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT75330.14170623486740883No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT73070.13745485970744145No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA71660.1348024530810901No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA68530.12891448659848037No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT67250.12650662810079974No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG66620.12532151024647253No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA66130.12439975191532918No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA64530.12138992879322835No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT64400.12114538066455767No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA64340.12103251229747887No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT63870.12014837675536176No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGAACTGTAGGCACCATC63860.12012956536084864No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAACTGTAGGCACCAT63050.1186058424052851No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG61800.11625441809114384No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT61080.11489999768619846No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT59100.11117534157259872No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAACTGTAGGCA58730.11047931997561292No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT57170.10754474243156462No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC56750.10675466386201315No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA56520.10632200178821118No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA56290.10588933971440917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG156500.069.365554
AGTAGGT246450.069.337167
GTAGGTT246050.069.321888
TTGGCGA142200.069.1542717
GCACGTA157550.069.1253746
TAGGTTG246450.069.0957649
GAGGTAG539450.069.0912252
ATTGGCG154500.069.0849816
GTAGTAG485150.069.0683445
GTTGTAT260900.069.0654812
CGTAAAT158050.069.0603949
GTTGACA205000.069.0028418
TGTATAG345650.068.9886714
GGTAGTA488100.068.980734
TAGTAGG247500.068.973616
TATAGTT307450.068.9665816
GCTGGCA57000.068.9466310
AGCTTAT343300.068.902212
GCTTATC343450.068.881013
AGATGCT57350.068.832266
Produced by FastQC (version 0.11.5)