FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5148335
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC633091.2296985336035826No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT359960.6991775010755904No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA296830.5765553329377362No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG286580.5566459836044081No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA246240.4782905541306072No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC234010.4545353012187435No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC200960.3903397894659147No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT198960.3864550383764848No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG196830.38231777846624204No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC194620.378025128512422No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC184800.35895100066332125No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA177420.34461626914332494No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT175020.33995456783600914No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA170130.330456351422353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160560.31186781745943104No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC149120.28964704122789214No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG147450.2864032740682182No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG142030.2758755986158632No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA135020.2622595460474114No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG130170.252839024655544No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT116190.2256846145404291No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA98260.1908578210236902No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG97830.19002259953946277No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA93000.18064092565848958No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG87200.16937514749914293No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG83090.16139198401036453No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT81030.15739069038825174No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT78870.15319515921166746No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC78300.15208800515117996No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA75180.14602779345166933No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC74010.14375521406435285No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC72780.14136609214435347No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA72640.14109415956809337No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC70280.1365101532825661No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG68000.13208153704061606No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA67850.1317901807089088No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA65650.12751695451053593No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT64520.12532207014500804No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA63720.12376816970923608No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA62860.12209772674078125No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA62290.12099057268029373No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT59600.11576558246501054No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT58710.11403686823021424No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT57270.11123984744582471No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG55780.10834570788419945No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT55750.108287436617858No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC54840.10651987487216741No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA53890.10467461810468823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT249650.069.5265058
AGTAGGT251050.069.417577
CAGCACG144850.069.4112854
GTAAGGT113950.069.316142
GTTGACA206550.069.2957918
GTTGTAT260250.069.29053512
TTGGCGA132600.069.2778717
GTAGTAG500250.069.277575
TAGGTTG250750.069.277339
TGTATAG343550.069.1843714
AGCTTAT336050.069.179522
GAGGTAG552500.069.162042
TAGTAGG251400.069.113476
GGTAGTA503550.069.0876544
TGTTGAC225400.069.07442517
GCTTATC336500.069.0135353
AGTAAGG114100.069.0078051
TGAGGTA542600.068.994451
TAGTTTG161150.068.990179
GCACGTA146200.068.985116
Produced by FastQC (version 0.11.5)