FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326051
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC330430.6204033720292952No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT270260.5074303644482563No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG200350.376169886469356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190750.3581452749889177No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA168470.31631315584473374No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA155430.2918297252504717No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG126410.23734282679606336No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA123050.23103421277790992No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT119070.22356150926831153No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC119030.2234864067204764No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC115830.21747820289366362No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC110490.2074520127576698No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC105490.19806419427827485No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT93180.17495138518200445No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA90180.16931869409436748No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC85240.16004352943672526No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA83660.15707697879723645No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG81750.15349083213810757No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG81660.15332185140547847No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA81660.15332185140547847No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG72010.1352033617402462No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT64950.12194776204734051No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG62060.11652160296625022No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA57600.10814766888262992No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA54950.10317212508855059No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG54060.10150109339921828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT200750.069.4312748
AGTAGGT202200.069.210287
TAGGTTG201350.069.172239
TGAGGTA423800.069.107381
GTTGTAT205700.069.0710712
GGTAGTA394600.069.067434
GAGGTAG433750.069.054512
CAGCACG105900.069.031444
GTAAGGT74900.068.822342
TGTATAG272500.068.8211114
GTAGTAG394250.068.791445
AGCTTAT230300.068.744382
GTATAGT272400.068.705715
TAGTTTG121600.068.694069
TAGTAGG202950.068.678636
AGGTTGT240250.068.663610
TTGGCGA96500.068.6492617
GCTTATC230750.068.640643
GTTGACA136100.068.62891418
CAGTGGC74500.068.580565
Produced by FastQC (version 0.11.5)