FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5479775
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC305840.5581251055015944No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT276050.5037615595530839No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG270880.4943268656103581No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA211330.38565452048669885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201860.3683727890287466No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA176800.322640984346985No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG158740.28968342678303394No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT144260.26325898417362026No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC139060.2537695434575325No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA129090.23557536577687951No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC129050.23550237007906347No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC124490.22718086052803266No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT116000.2116875236665739No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC109640.20008120771382037No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA99680.18190527895762143No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG94660.1727443188817059No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG94290.17206910867690736No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG89330.1630176421477159No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC85350.15575457021501796No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA78850.14389276931990821No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA74820.13653845276494017No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA72700.13266968078068897No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG71940.13128276252218385No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT67070.12239553631307855No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC55350.10100779685297298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT260450.069.34658
CAGCACG150100.069.2932744
AGTAGGT261200.069.2545557
GTTGACA199000.069.1856618
AGCTTAT326800.069.168152
GCTTATC326250.069.1667563
TGAGGTA558300.069.0914461
TAGGTTG261400.069.081089
GGTAGTA521450.069.026294
GTTGTAT272700.069.003312
TGTATAG360800.068.9833314
GTAGTAG519200.068.981665
GAGGTAG572350.068.977722
AGGTTGT309600.068.9063110
TTGGCGA136150.068.8874417
GCACGTA151350.068.8828356
TAGTAGG262750.068.873916
ATTGGCG147650.068.83131416
GATAGGC18050.068.8287915
GTATAGT360400.068.8171415
Produced by FastQC (version 0.11.5)