FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4811679
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC640511.3311569620500454No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA344180.7153012493144285No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG331930.6898423606396021No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA240330.49947222165069616No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG234470.4872935206193098No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC220700.4586756514721784No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT219970.4571585095348214No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC201200.41814925725510776No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT185710.3859567523103682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184230.3828809029031238No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC155030.3221952254088438No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC138630.28811148873397413No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA129140.26838864354833314No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG115940.24095539207831612No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA110400.22944173956741507No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT103490.21508084807818642No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC103350.2147898893504741No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG100560.20899149756249324No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA90010.1870656791527448No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG89840.186712372126237No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT81150.16865214824180913No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG74270.15435360505137605No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA66650.13851713715732078No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT66490.13818461289707812No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG62490.12987150639101236No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG62180.12922724063679228No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA57910.12035299944156708No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA57530.11956325432349083No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC52930.11000318184151521No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT52710.10954596098368158No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT50440.10482827304148926No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT48320.1004223265932744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT266550.069.434288
CAGCACG138350.069.429934
AGTAGGT268000.069.358927
TAGGTTG266800.069.329879
GAGGTAG545850.069.230642
TTGGCGA125400.069.2086317
GTTGTAT273900.069.20849612
ATTGGCG136200.069.1942416
GTAGTAG492750.069.112895
GGTAGTA495950.069.104414
TGTATAG350950.069.100414
GTTGACA161050.069.0795518
TGAGGTA532100.069.044161
TAGTAGG267850.069.006556
TATAGTT313250.068.985316
TAGTTTG141350.068.970669
GCACGTA139800.068.910066
AGCTTAT256700.068.882912
GCTGGCA56050.068.8008910
TAGTAGT141550.068.799766
Produced by FastQC (version 0.11.5)