FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4836474
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC488871.010798362608793No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG259910.5373956316109629No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA257950.5333430925091296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186760.3861490829889709No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA184990.3824893920653766No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG181280.37481851447976355No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC176520.36497663380388273No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT170410.3523434634405147No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC151380.3129966169568988No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT130790.27042428016774206No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC124080.25655053660993526No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC116570.24102269545954344No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA106770.22075999995037707No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA94360.19510081104540208No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG90520.18716114260099403No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC86400.17864253999918123No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT78140.161563982355741No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG70700.14618087474470037No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG66250.13697995688594625No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT64310.1329687702239276No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA63260.1307977671336598No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT55470.11469099182586323No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG54230.11212714055735647No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG53540.11070048138375188No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG50890.10522128310831404No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT50340.10408409101341183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT249950.069.4272468
TAGGTTG250500.069.274819
AGTAGGT251600.069.264017
GAGGTAG516850.069.217562
GTTGTAT264950.069.20812
GGTAGTA465200.069.184524
GTAGTAG462600.069.1497345
TAGTAGG251050.069.125186
GTTGACA158400.069.1071518
ATTGGCG134200.069.07674416
CAGCACG137400.069.072534
TGTATAG339800.069.0714214
AGGTTGT300650.069.0444710
TGAGGTA505950.069.028671
AGCTTAT253450.069.00912
TATAGTT303100.069.0080516
AGATGCT53950.068.9515846
AAGATGC54050.068.8887565
TTGGCGA125250.068.87182617
GTATAGT339650.068.8450615
Produced by FastQC (version 0.11.5)