FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5134639
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC527521.0273750501252377No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA329170.6410772013378155No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG309330.6024376786761445No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA260480.5072995394612941No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG250300.48747341341815853No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC243910.47502852683509006No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT220030.4285208755669094No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC201290.392023665149585No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC183630.35762981584489195No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT180760.35204032844373284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170670.33238948249331646No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA152200.29641811235414994No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC133710.260407791083268No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA122140.23787456138591243No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG122120.23783561025419706No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT108190.21070614701442497No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG83850.16330261971679022No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG80540.1568562074178925No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC80370.15652512279831163No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA71820.1398735139899806No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT66600.1297072686122627No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC64820.12624061788959262No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT63740.12413725677696133No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT62660.12203389566433004No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT60190.11722343089747887No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA57690.1123545394330546No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA54600.10633658958302619No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG54220.1055965180804337No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC53870.1049148732754143No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG51670.10063024878672094No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT51360.10002650624513232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT252950.069.4903268
AGTAGGT253900.069.395727
TAGGTTG253800.069.2024469
GGTAGTA472600.069.1967544
GAGGTAG521350.069.183582
TATAGTT303200.069.0908216
GTTGTAT262100.069.05578612
TAGTAGG254600.069.040666
AGCTTAT251550.069.029162
GTAGTAG471350.069.016475
TGTATAG341850.068.998114
CAGCACG121950.068.898624
GCTTATC251600.068.862443
AGGTTGT301700.068.8168810
TGAGGTA511950.068.814951
GTTGACA161800.068.80150618
GCTGGCA61400.068.79150410
GTATAGT342300.068.7335915
CAAGATG61500.068.680314
TTGGCGA112150.068.6181117
Produced by FastQC (version 0.11.5)