FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4547996
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC640501.408312584267884No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG266690.5863901375462951No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA242930.5341473475350462No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC217170.47750701627705916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180010.39580069991266487No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA175390.38564237963269976No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG169240.37211994029898005No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC166500.3660953087909488No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT155770.34250250000219873No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA135470.2978674563478068No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC134560.295866575080541No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT125520.2759896886452846No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC117940.2593230073201472No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC94180.20708021730889825No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG92590.20358417201774143No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG92370.20310044248060022No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA92230.20279261459332856No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG91900.20206702028761678No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT88030.19355777797517854No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT81790.17983744928535556No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA68230.15002211963247108No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA63180.13891832798445733No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT60190.13234400382058384No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG57700.12686906496839487No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG57310.12601154442528095No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG53770.11822789641855445No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT51060.1122692280292243No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA47010.1033642070045796No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA46480.10219885857419399No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT45800.10070369455030302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGCT49200.069.6351856
CAGCACG125400.069.544324
GTAGGTT223000.069.393738
AGTAGGT223650.069.332887
TAGGTTG223150.069.315739
GTTGTAT232400.069.2522112
GTTGACA153700.069.1476318
AAGATGC49650.069.145025
ACAGTGG78400.069.142724
GAGGTAG462000.069.142452
TGTATAG296900.069.1414114
GGTAGTA416900.069.092644
GTAGTAG414100.069.0781255
AGCTTAT244950.069.033622
ATTGGCG123500.068.9699616
GCTTATC244950.068.962193
TAGTAGG224100.068.960186
TGAGGTA449800.068.9127661
TTGGCGA114050.068.88544517
TATAGTT264500.068.87868516
Produced by FastQC (version 0.11.5)