FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6166487
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC588720.9547088966537998No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA314690.5103229764369892No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG303000.4913656673564705No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA256150.4153904808361714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233620.37885428121392295No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC217470.35266432897693617No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG213350.3459830532359835No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC197670.32055528536750344No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT194570.31552811187309726No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT184150.298630322256416No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC154870.2511478577673155No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC152590.24745045274562324No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC137370.22276865255695827No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA135290.2193955813090987No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT113930.18475673426377126No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG111100.18016741136403921No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA108930.1766483899179549No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG86970.14103654155112952No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA86760.14069599108860523No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT84320.13673911904784686No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG82820.13430661574410196No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG78140.12671720543641785No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG75090.12177111538546988No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG75070.1217386820080866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT210350.069.522798
AGTAGGT211500.069.3929447
GTTGTAT214450.069.3870812
TAGGTTG211100.069.242639
GGTAGTA394250.069.201424
CAGCACG116900.069.123284
GAGGTAG433150.069.119432
TAGTAGG211650.068.9987956
GTATAGT277400.068.9827515
TGTATAG278900.068.9128914
GTTGACA135500.068.8297718
GTAGTAG393450.068.808465
GCTTATC217400.068.800793
TATAGTT249450.068.7860516
AGCTTAT217600.068.753632
AGGTTGT249550.068.6850210
GGCTATG29400.068.56415616
TGAGGTA426650.068.5370941
TTGGCGA107550.068.5277317
GGGCTAT32700.068.3866915
Produced by FastQC (version 0.11.5)