FastQCFastQC Report
Tue 7 May 2019
HCGVJBGXB_n01_AL206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5060650
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC655321.2949324691492201No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA405560.8013990297689032No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG381880.7546066216790334No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA326090.6443638663017597No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG270550.5346151186112456No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC270010.5335480620078449No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT245850.4858071591593965No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC219750.43423275666169364No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT213020.42093406973412506No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC190480.37639433669587896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186700.36892494047207375No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC171620.3391263968067343No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC167890.331755802120281No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA163710.3234959935976604No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT152910.30215486152964544No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA146090.28867833183484337No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG134410.2655982927094346No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA111390.22011006491261No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG101870.20129825220080427No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG100390.1983737266951874No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT90810.17944335213855928No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG89720.1772894786242874No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG86380.17068953592917904No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT76430.15102802999614676No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA76010.1501980970823906No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT75080.14836038848764485No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA71910.14209637101953307No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC70950.14019938150237618No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG66320.13105035914358826No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA64410.1272761404167449No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT60430.11941153804353197No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT60310.11917441435388736No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC59470.11751454852637506No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA59010.11660557438273739No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT57610.1138391313368836No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG56820.11227806704672325No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA55510.10968946676810291No Hit
TCAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGA53760.10623141296078567No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAA53030.10478891051544763No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT52550.10384041575686917No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA52360.10346496991493188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT291550.069.5221568
CAGCACG171500.069.460434
AGTAGGT293550.069.418057
GAGGTAG616550.069.327562
TGCAGGT50850.069.3013915
GTTGTAT294100.069.3001312
TAGGTTG292500.069.296369
GTAGTAG562900.069.2884455
TTGGCGA156700.069.2776917
ATTGGCG169500.069.2490816
TGTATAG385950.069.2370114
GTATAGT384450.069.21586615
GCACGTA172300.069.1981666
GGTAGTA567400.069.195344
TAGTAGG293950.069.156916
GGATAGG42800.069.0901914
AGCTTAT274250.069.060142
TTTGTGC159800.069.0486112
ACAGTGG92900.069.011544
TATAGTT345200.069.0089716
Produced by FastQC (version 0.11.5)