FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32765275
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23907347.2965479459580305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT82850.042.9719439
TCGGAGA80950.042.6472478
GCTCGGA78300.042.3600736
CTCGGAG100550.032.6825457
GGCTCGG161650.018.3289895
TGGGCTC163200.016.6118983
GGGCTCG156600.015.1402474
TATACTG157100.012.8043535
TACACTG121800.012.5802495
CTACACT110450.012.5110864
CTAGGAC59600.012.3501323
CGTGGGC153300.012.0449471
CTATACT116650.011.4096794
TAGACAG161350.011.3869035
GTATTAG121150.011.2051081
GTATAGG91200.011.2036541
TAGGACA90400.011.0219714
TAGGACT87000.010.9511364
CTAGACT73400.010.8994294
CTAGCCT77750.010.8508734
Produced by FastQC (version 0.11.5)