FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_PR8_P1_ovgr_2ng_KJ.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_PR8_P1_ovgr_2ng_KJ.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747821
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG50600.28950333014650814No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT45920.26272713281279947No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC43650.24973953282401345No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC40600.23228923327960926No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG34080.19498564212239125No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA32710.18714731085162611No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA28420.16260246329572653No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT26950.1541919910562924No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT25820.14772679811033282No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT25040.1432640985547147No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT23560.13479641221841365No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT23500.1344531276372123No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT22860.13079142543773076No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG22590.12924664482232448No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA22500.12873171795052238No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT22440.12838843336932101No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA21680.1240401620074367No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT21550.123296378748167No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT21510.12306752236069941No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA19350.11070927743744925No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA19100.10927892501577678No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC18530.10601772149436356No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA18450.10556000871942836No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA18100.10355751532908691No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT18060.10332865894161931No Hit
CAATAGATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTG17840.10206994881054754No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA17550.10041074000140748No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA17500.10012466951707297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG29300.087.355033
GAGCGAA3850.084.809291
AGCAAAA35100.072.7282942
AGCGAAA7700.070.674411
GCAGGGT22750.067.559379
AGCAGGG31550.062.9625558
AAGCAGG49500.058.5849347
CAAAAGC46000.056.4230274
ACAAGGG8350.048.6219867
TAAACTA5300.046.50875
CCTAGCG951.9219242E-645.78875145
AAGGTCG3850.043.3110059
GCAGGGG11400.042.608989
GTAGTAA10000.042.084251
TCGTACT5100.041.2378162
TTTAAAC6500.039.0423133
GAGCAAA69350.038.9213641
CAAGGGT10400.038.340748
AGGGTGT7950.038.301286
GTAAACA11050.038.0536964
Produced by FastQC (version 0.11.5)