FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_neg.4.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_neg.4.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62140
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1697427.31573865465079No Hit
CGTGGGCTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTG5100.8207273897650467No Hit
CTTATACACATCTGACGCTGCCGACGACTAGTCGAGTGTAGATCTCGGTG2780.4473768908915352No Hit
CGTGGGCTCGGAGATGTGTATAAGACTGTCTCTTATACACATCTGACGCT900.14483424525265529No Hit
CGTGGGCTCGGAGATGTGTATAAGAGCTGTCTCTTATACACATCTGACGC710.11425812681042807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA1250.0127.597876
CTCGGAG1200.0126.872897
CGGAGAT1200.0126.872899
CGTGGGC1350.0123.815541
GGGCTCG1350.0123.516464
TCGGAGA1250.0121.7979668
GGCTCGG1350.0118.146175
GTGGGCT1600.0104.217012
TGGGCTC1750.095.284133
CACAATG406.614682E-572.4987957
TCTAGAG350.0035510162.1418233
TAACCTG350.0035510162.1418235
TCTACAG400.006024698754.3740923
AACCTGA400.006024698754.3740926
TTCTACA450.00959757748.3325272
CCAGGCA450.00959757748.3325273
TATAAGC950.024.42064515-19
GAGATGT1500.024.16626410-14
GTATAAG1550.022.45123915-19
GTGTATA1750.021.54249815-19
Produced by FastQC (version 0.11.5)