FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_9109.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_9109.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19594236
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266880.1362033202008999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA78700.087.607396
TCGGAGA86650.082.413688
CGGAGAT90900.079.0389949
GGCTCGG83100.072.3250055
CTCGGAG101600.066.43347
GGGCTCG75550.066.119864
CGTGGGC72450.046.68271
TGGGCTC220550.024.2635973
GTGGGCT153500.023.7226432
GCCGACC62600.022.004246145
GATCGGA19800.021.6033846
GACGACA60600.019.859241145
TGGCTCG43950.017.8160744
CCGTATA69550.017.407907145
TGCTCGG45450.015.9514835
GCTCCGA27150.015.7549536
TACACTG158300.015.75478655
TATACTG211150.015.3823215
GTATAGA140550.015.078581
GTATTAG136600.015.0364111
Produced by FastQC (version 0.11.5)