FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_8404.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_8404.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18603871
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA111300.083.638796
TCGGAGA124000.079.164238
CGGAGAT126150.078.446779
CTCGGAG126300.073.3026357
GGCTCGG122450.067.023255
GGGCTCG105000.066.908744
CGTGGGC116100.041.0696141
TGGGCTC207900.035.0868453
GTGGGCT155750.033.3926432
GATCGGA30500.022.8196646
TATACTG183500.016.6730185
GCTCCGA45350.016.1466316
TCCGAGA45850.016.1284588
TACACTG137550.015.4961795
ATCGGAG44300.015.3835347
CTACACT108950.015.3055514
GTATAGA129450.015.0251991
CTATACT130050.014.6062694
GTATTAG145050.014.2598381
CCTACAC92050.014.02158453
Produced by FastQC (version 0.11.5)