FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_8404.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_8404.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16837617
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206070.12238667740215257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA75900.082.9115456
CGGAGAT86650.076.5569159
TCGGAGA86700.076.262358
CTCGGAG89450.069.8650747
GGCTCGG83100.067.5269245
GGGCTCG74600.064.629034
CGTGGGC80000.041.549711
TGGGCTC190400.026.7340813
GTGGGCT138350.025.6380352
GATCGGA22750.018.1647956
TATACTG177050.016.0109795
TCCGAGA33500.015.149138
TACACTG141750.015.1392975
CTATACT121050.014.4942354
TAGACTG105550.014.4930985
GTATAGA120200.014.2495151
CTACACT104000.013.8728134
CCTACAC84800.013.5955373
GCTCCGA33100.013.1419656
TACACCG36450.013.12754255
Produced by FastQC (version 0.11.5)