FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_6852.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_6852.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17351200
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174430.10052907003550186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA45500.068.675556
TCGGAGA52450.062.754218
CGGAGAT54150.062.123129
CTCGGAG56950.054.9950757
GGCTCGG53150.052.9259265
GGGCTCG46850.050.449494
CGTGGGC54950.030.2427311
CCGACGC107350.015.66804145
TATACTG210550.015.5985045
GCCGACC32550.015.368336145
GTCCTAG78650.015.2243381
GTGTAGG81100.015.21182251
TACACTG155950.014.969615
GCTCCGA20550.014.8174596
GTGGGCT128450.014.6819352
GTATTAG123650.014.6723311
GTATAGA133800.014.5898021
CTATACT131750.014.2527254
GTACTAG59700.013.979021
TGGGCTC195800.013.9241593
Produced by FastQC (version 0.11.5)