FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_6751.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_6751.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19061280
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223850.11743702416626796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA87800.074.811696
GGCTCGG91350.065.15845
CTCGGAG102500.063.7281077
CGGAGAT109300.062.88059
TCGGAGA111400.061.7603848
GGGCTCG82250.058.0006184
CGTGGGC88850.036.757671
TGGGCTC176350.028.3290463
GTGGGCT134600.026.0829772
TACACTG130450.015.7837455
TATACTG172300.015.5686975
GTATAGG86950.014.5235411
GTCCTAG67850.014.440321
CTATACT148950.014.16434
TCTATAC142950.013.644463
GATCGGA28750.013.6173226
GTGTAAG110400.013.345041
GTCTAGG73750.013.1866861
GGACCGT32050.013.1200586
TACACCG57800.013.0449165
Produced by FastQC (version 0.11.5)