FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_6331.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_6331.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17223509
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188600.10950149589145858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA53650.060.4058046
CGGAGAT63950.054.0765089
TCGGAGA63000.052.936098
CTCGGAG65050.048.927367
GGCTCGG61950.046.3442655
GGGCTCG55200.044.131244
CGTGGGC65150.024.0629141
TATACTG193400.016.2320585
TGGGCTC170650.015.8911973
TACACTG142500.014.7036495
CTATACT131750.013.6473074
GTGGGCT124450.013.638012
CTACACT110500.013.5160834
GTATAGG69900.013.2905121
CCTACAC83700.013.0810563
CTAGGAC71250.012.8226273
GTGTAGG81800.012.6879551
TATACAG167800.012.6595365
ATACTGT252950.012.6112726
GTGTTAG121100.012.5858941
Produced by FastQC (version 0.11.5)