FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_6119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_6119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16151757
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197780.12245107451777537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA53650.078.5127946
CGGAGAT64550.070.532959
TCGGAGA63250.070.377948
CTCGGAG66150.063.4568827
GGCTCGG59200.059.885435
GGGCTCG50700.058.77224
CGTGGGC59800.034.3443371
GCCGACC36500.019.465395145
GTGGGCT116450.018.4990772
TGGGCTC180650.017.9807473
GATCGGA15400.017.4186736
TATACTG192450.015.93516355
CCGACGC72800.015.435841145
TCCGAGA25950.014.5276268
TGCTCGG37150.014.4413225
GTGTAGG72900.013.7379341
TACACTG144800.013.4183865
CTATACT118850.012.932274
GTATAGG66350.012.7971961
GTGTACG21550.012.7969431
Produced by FastQC (version 0.11.5)