FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_4340.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_4340.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17792668
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185940.10450372029647267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA96750.0100.787346
TCGGAGA109150.093.719918
CGGAGAT114700.089.753949
GGCTCGG98200.087.4127665
CTCGGAG112850.086.535557
GGGCTCG83550.084.953624
CGTGGGC78300.060.8002471
TGGGCTC214050.035.3653953
GTGGGCT153450.034.2697452
GCTCCGA28450.027.7765886
TGGCTCG44500.025.7420254
TCCGAGA30950.024.829838
TGCTCGG42600.023.8261095
GATCGGA22550.023.791396
CTCCGAG32700.019.9536937
ATCGGAG30800.019.3015297
GCCGACC48950.018.661442145
CGGCTCG24700.017.6116474
TATACTG197650.016.9098115
TACACTG147800.015.9420295
Produced by FastQC (version 0.11.5)