FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_31013.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_31013.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18161376
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231190.12729762326378793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA81650.091.901866
CGGAGAT93050.083.1339959
TCGGAGA92800.082.889668
CTCGGAG96900.076.090657
GGGCTCG73250.076.014654
GGCTCGG87100.075.080955
CGTGGGC77850.048.0078471
GTGGGCT149200.027.2726842
TGGGCTC212850.027.2183063
GATCGGA18700.020.1605266
TCCGAGA30500.019.9669828
GCTCCGA28450.019.8770756
TGGCTCG41700.016.864734
TGACGCG37150.015.416879145
CTCCGAG40050.015.3868797
TATACTG201400.015.3712695
GCCGACC54450.015.311867145
TACACTG144800.015.0709025
CTATACT129800.014.9693794
GTGTAGG85500.014.9386091
Produced by FastQC (version 0.11.5)