FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_31013.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_31013.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17839283
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA66900.081.928046
TCGGAGA73900.078.385038
CGGAGAT75650.077.0502859
CTCGGAG79650.068.903447
GGCTCGG77200.063.2966545
GGGCTCG64450.062.7701344
CGTGGGC67900.039.3311231
GATCGGA19500.020.820466
TGGGCTC212600.019.8836333
GTGGGCT145900.019.6375892
GCTCCGA26200.016.8797286
TATACTG211850.016.7689445
TGGCTCG42150.016.3405914
CTACACT116700.016.0284164
TACACTG160650.015.795215
GCCGACC49100.015.060681145
CTATACT134350.014.3544234
GTATAGA129950.014.3520891
CCTACAC87500.013.7559483
TCTATAC131600.013.6642643
Produced by FastQC (version 0.11.5)