FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30903.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30903.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16515230
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168420.10197859793657127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA103350.096.6669856
CGGAGAT124150.086.893069
TCGGAGA122100.086.5706568
GGCTCGG105800.084.1498645
CTCGGAG118800.084.033097
GGGCTCG91700.082.147594
CGTGGGC92150.056.3899541
TGGGCTC191200.041.526073
GTGGGCT137350.039.5558622
GATCGGA28450.023.4447146
TCCGAGA38300.020.2541458
TGGCTCG53350.020.1129574
GCTCCGA40300.019.7891466
ATCGGAG39150.018.147937
CTCCGAG41550.016.5762167
TATACTG173600.016.4128325
TGCTCGG54400.016.1260195
GTGTAGG76350.015.9692421
CTATACT119950.015.0503884
ACGGAGA49850.014.8341918
Produced by FastQC (version 0.11.5)