FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30900.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30900.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472112
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA45000.058.4825746
CGGAGAT57600.049.2133069
GGCTCGG49300.048.381965
CTCGGAG53350.048.1059887
GGGCTCG43000.047.546584
TCGGAGA61000.045.4010548
CGTGGGC52300.026.642811
TGGGCTC92900.023.2592183
GTGGGCT75450.020.1883052
TACACTG71800.015.8529355
CCTAGAC34100.014.2467253
GTATAGG53400.014.1342451
TATACTG105800.013.9790935
TAGACTG53500.013.8223185
TATACCG28400.013.7850995
TACACCG37650.013.4793065
CTACACT62600.013.3187084
CTAGTAC30100.013.2492353
CTATACT79000.013.2152084
CCTATAC50500.013.2096423
Produced by FastQC (version 0.11.5)