FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30890.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30890.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22390347
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268320.11983735669661574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA112900.091.9573446
TCGGAGA127350.085.5642858
CGGAGAT129700.084.0135969
GGCTCGG116900.078.453855
CTCGGAG133750.077.296747
GGGCTCG103500.075.02284
CGTGGGC104400.050.609951
TGGGCTC267150.030.4257373
GTGGGCT190900.029.600152
GATCGGA28300.023.0565036
GCTCCGA40500.019.6913286
TCCGAGA44100.017.097298
TGGCTCG63800.016.3638674
CGGCTCG36150.016.0444874
CTACACT137200.016.0115224
TACACTG179700.015.8152585
GCCGACC67350.015.608282145
ATCGGAG41100.015.3466227
TGCTCGG56950.015.2765695
CTCCGAG46850.015.165337
Produced by FastQC (version 0.11.5)