FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30887.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30887.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001777
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG225920.10757184975347563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA77800.085.918266
TCGGAGA86050.080.629548
CGGAGAT89950.076.5690849
CTCGGAG92000.071.474567
GGCTCGG85100.069.091965
GGGCTCG74650.066.916294
CGTGGGC80150.042.197151
TGGGCTC245550.022.087553
GTGGGCT167850.021.4777832
GATCGGA19500.018.2177876
GCTCCGA29950.016.9447576
TCCGAGA35750.015.615218
TATACTG223900.015.5102085
TGGCTCG46200.015.2220154
GCCGACC53500.014.906161145
TACACCG31650.014.8893545
CTATACT156150.014.857664
GTATTAG157400.014.7552291
GTATAGG85500.014.4305721
ATCGGAG29150.014.4251887
Produced by FastQC (version 0.11.5)