FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30886.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30886.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19450310
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211040.10850212670132249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA81000.085.656876
TCGGAGA91850.078.6945348
CGGAGAT94650.077.8222669
CTCGGAG95250.072.7648247
GGCTCGG88150.069.908985
GGGCTCG77200.065.551794
CGTGGGC84050.039.288231
TGGGCTC232300.022.9420683
GTGGGCT157200.022.93322
TATACTG236150.017.4073035
GATCGGA22400.017.1539126
GCCGACC58950.016.602777145
TACACTG167950.016.3604625
CTACACT126050.015.9324794
TGGCTCG54700.015.3750694
CCGACGC100400.014.803041145
TCCGAGA34550.014.6885388
CTATACT145200.014.5801724
GTGTAGG89650.014.5717251
GCTCCGA33400.014.3262566
Produced by FastQC (version 0.11.5)