FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30886.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30886.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20444581
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295160.14437077482781377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA144400.0107.645236
TCGGAGA161650.0100.91088
CGGAGAT163200.0100.840869
CTCGGAG161200.094.986257
GGCTCGG144950.093.982235
GGGCTCG127450.090.334844
CGTGGGC108900.070.180591
GATCGGA22550.042.4389466
TGGGCTC287200.041.5058983
GTGGGCT202300.040.589732
GCTCCGA33850.030.8418946
ATCGGAG36000.029.603757
TCCGAGA40100.028.7464078
CGGCTCG26700.028.5116714
TGCTCGG50300.027.0973645
TGGCTCG59000.026.9114274
CTCCGAG46500.024.0104147
GGATCGG24350.023.2237765
GCACGGA38050.021.7213786
TCGGACA24850.021.589188
Produced by FastQC (version 0.11.5)