FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30849.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30849.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19167437
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA106800.032.584586
CGGAGAT127250.029.284399
CTCGGAG123000.028.0567387
TCGGAGA134300.026.6136238
GGGCTCG104800.024.3516944
GGCTCGG124000.024.2644065
TGGGCTC116100.023.4209583
TATACTG129950.017.2953225
GTGGGCT123300.015.9429312
TACACCG135900.015.7912145
TATACCG79450.015.6043435
TACACTG92900.014.98403555
CTATACT121150.014.4823734
GTATAGA144400.014.4247041
TAGACAG94500.014.4234555
GTATAGG85050.014.2506391
GTGTAGG108950.013.789081
TCTATAC140050.013.4615743
CTAGACT41550.013.2614314
CTACACC190700.013.2305034
Produced by FastQC (version 0.11.5)