FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30848.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30848.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17630959
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA95100.050.009736
CTCGGAG111350.042.971817
GGCTCGG98150.042.9162675
CGGAGAT123900.042.305519
GGGCTCG88150.040.6303524
TCGGAGA130200.039.2561848
TGGGCTC117300.032.5759243
GTGGGCT109600.023.2965972
CGTGGGC122900.020.313571
TATACTG154000.016.3830455
TACACTG108750.016.1999285
CTATACT136550.014.6542424
TACACCG87100.014.6497645
TATACCG71500.014.4999375
CTACACT101900.014.0875984
GTATAGG92300.013.9171821
TCTATAC154350.013.3412293
GTATAGA159900.013.2983841
GTGTAAG115100.012.7366671
CCTATAC95300.012.6298843
Produced by FastQC (version 0.11.5)