FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30844.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30844.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15152899
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197340.13023250534435687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA74500.079.895956
TCGGAGA89900.071.2896048
CTCGGAG87800.068.7009057
CGGAGAT94400.068.1982359
GGCTCGG79800.067.049085
GGGCTCG70750.062.0997434
CGTGGGC75300.038.1681631
TGGGCTC153400.030.8190273
GTGGGCT112650.027.8811742
GATCGGA24650.016.4705856
TATACTG149550.016.1919575
TGGCTCG49200.015.9148414
GCTCCGA36100.015.8656476
TACACTG108100.015.5597155
GTACTAG49200.015.19403651
TCCGAGA38100.014.6520838
GTATAGG69650.014.17159751
GTGTAGG71050.014.0966551
ATAGGAC69000.014.0816123
TCTATAC111100.014.0320483
Produced by FastQC (version 0.11.5)