FastQCFastQC Report
Thu 2 May 2019
HCGGGBGXB_n02_MG_30830.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30830.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15134720
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173110.1143793872631935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA62100.084.057816
CGGAGAT74750.074.972859
TCGGAGA74400.073.7661748
CTCGGAG73750.070.28737
GGCTCGG67000.068.821225
GGGCTCG57850.066.548954
CGTGGGC58350.043.532651
TGGGCTC163350.024.8580063
GTGGGCT117100.023.1663992
GATCGGA21950.019.1571376
TATACTG164450.018.0754875
TACACTG123550.015.9612245
GCTCCGA27450.015.0546156
CTATACT110900.014.9711094
TATACCG23350.014.5932155
TACACAG150300.014.3264145
CGGCTCG24300.014.3213854
GTATAGG65500.013.8502191
GTCCTAG62450.013.7131581
GTGTAGG69600.013.6599781
Produced by FastQC (version 0.11.5)