FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n02_MG_30786.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30786.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15631397
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198410.12693043366501405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA67900.085.419066
TCGGAGA77700.077.537268
CGGAGAT80050.075.804429
CTCGGAG79150.071.811537
GGCTCGG74150.071.081945
GGGCTCG65150.066.881464
CGTGGGC67450.042.9263951
TGGGCTC187550.024.6657183
GTGGGCT125900.024.5416242
GATCGGA16000.023.5623156
ATCGGAG25550.017.308847
TGGCTCG39250.017.1786484
TACACTG131500.016.9257765
TATACTG181850.016.784365
CTATACT115800.015.5270454
CGGCTCG21900.015.228584
CTACACT101750.015.1771744
TACACCG23850.014.8951095
GTATAGG65050.014.6136091
TCTATAC115850.014.5205183
Produced by FastQC (version 0.11.5)