FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n02_MG_30785.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30785.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20296564
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292780.14425101706870186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA93700.069.6378866
CGGAGAT114550.060.8856859
TCGGAGA114850.060.6005448
CTCGGAG123250.053.411427
GGCTCGG110850.051.93115
GGGCTCG93950.050.5474134
CGTGGGC114400.028.8011821
TGGGCTC201800.025.0804163
GTGGGCT151000.023.3940562
TATACTG192450.015.7471165
TACACTG144600.015.4929065
GATCGGA29650.015.4048886
CTACACT112050.014.9470194
CTATACT137450.014.8750634
GTATAGA149350.014.14061451
GTATAGG83700.014.0465631
GTGTAAG117500.013.7118371
GTATTAG144850.013.3774481
TACACCG59500.013.1598055
GTCTTAG101200.012.9801161
Produced by FastQC (version 0.11.5)