FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n02_MG_30783.TR5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n02_MG_30783.TR5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14789052
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG191240.12931187205238037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA86500.078.7025766
CGGAGAT104700.068.483069
TCGGAGA109900.065.243178
GGCTCGG91250.064.992435
CTCGGAG106250.063.731337
GGGCTCG81050.061.901944
CGTGGGC87300.038.572521
TGGGCTC148600.035.4745333
GTGGGCT114750.032.238242
GATCGGA26500.019.1510076
TACACTG101100.017.4977045
GTCCTAG52650.015.8516241
TATACTG138950.014.4010085
TCTATAC103450.014.2287453
GTATTAG113350.013.8935271
CTACACT80700.013.6559284
GTGTAGG74150.013.6043551
TACACCG44700.013.4621045
TGGCTCG54950.013.45809654
CTATACT104400.013.3337384
Produced by FastQC (version 0.11.5)