FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32765275
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22677306.921138308773542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGA59400.018.1618358
GCTCGGA60150.018.0584496
CTCGGAG62500.017.2610597
CGGAGAT63400.016.7876519
GGGCTCG64900.016.625134
GGCTCGG66650.016.2972875
TATACTG180200.014.1854815
CGTGGGC84550.013.0428811
TACACTG146900.012.7673915
GTCCTAG66300.012.4748381
CTATACT128800.012.2002124
GTATTAG131750.012.1698491
CTACACT121450.011.8653094
GTGTTAG119350.011.6105961
GTGTATA202900.011.5137651
GTGTAGA119800.011.5064231
TAGACAG182700.011.2961515
GTATAGA134850.011.1906661
GTATAGG83650.011.1883831
TAGACTG105100.011.0240565
Produced by FastQC (version 0.11.5)