FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_PR8_P1_ovgr_0.2ng_KJ.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_PR8_P1_ovgr_0.2ng_KJ.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501284
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG73210.2926896745831341No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT66940.2676225490588034No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA60030.23999673767552987No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC59540.23803774381477671No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC58380.23340012569544283No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT45410.1815467575853042No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG44810.17914798959254527No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT42320.16919310242259578No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT42000.16791375949312434No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT39460.1577589749904449No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT39260.15695938565952527No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA39060.15615979632860563No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT37100.14832382088559318No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC36310.14516544302846057No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA35510.14196708570478203No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA34540.13808907744982177No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT33850.13533049425814903No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT33510.1339711923955856No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA32340.12929359480970573No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT31710.12677488841730886No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA31500.12593531961984325No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA31140.1244960588241879No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA31010.12397632575909014No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA30350.12133768096705533No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA30100.12033819430340577No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA29610.11837920044265265No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA29230.11685998071390533No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG27550.11014343033418036No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA27200.10874414900507101No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA27010.10798453914069733No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT26380.10546583274830047No Hit
CAATAGATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTG26100.10434640768501298No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT26080.10426644875192101No Hit
GTACAAGGACTTCTTTCCCTTTTTTGTTCACATAAGAATTTTTCAGCTTT25760.10298710582244959No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG25040.10010858423113889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG38550.085.016333
AGCGAAA9650.081.912131
GAGCGAA3550.081.711021
AGCAAAA44050.072.91992
GCAGGGT28950.065.60769
AGCAGGG41400.061.6373188
GCAGGGG10550.061.1559879
TAAACTA7950.058.371535
AAGCAGG67450.053.3101437
CTATTCG9050.052.0674369
AAGGTCG3950.051.3880779
CAAAAGC65400.050.22374
GCGCGAA604.940282E-448.3456951
TCGATGG752.5526606E-548.33023145
ACAAGGG15000.046.397027
TTTAAAC10000.045.6802833
AGGGTGT13300.044.704426
TCTATGC19450.044.3624573
CTATGCT20050.043.0349044
TTTTTAA10950.042.3852651
Produced by FastQC (version 0.11.5)