FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_PR8_P1_ovgr_0.2ng_CW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_PR8_P1_ovgr_0.2ng_CW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745184
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG56470.3235761959770431No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT46160.2644993307295964No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC41110.23556255386251537No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC36640.20994920879402976No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA33590.1924725415772778No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT33370.1912119295157416No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA31000.17763169958010158No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT29950.1716151420136788No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT29430.16863551350459322No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT26550.15213295560811926No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA26210.15018473696756332No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT25750.1475489117479876No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA24360.13958413554100885No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA23110.13242156700955315No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA22680.1299576434347324No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT22380.12823862698718302No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT22190.12714991657040176No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC22190.12714991657040176No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG22030.12623310779837543No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA20800.11918514036342301No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA19990.1145437959550397No Hit
GTACAAGGACTTCTTTCCCTTTTTTGTTCACATAAGAATTTTTCAGCTTT19620.1124236756697288No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA19250.11030355538441791No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA19050.10915754441938501No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA18940.10852723838861689No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA18900.10829803619561032No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT18860.10806883400260373No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT18520.10612061536204778No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG18320.10497460439701488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG30100.091.773223
AGCAAAA34150.078.978662
AGCGAAA5700.077.595651
GAGCGAA2900.075.007671
GCAGGGT22500.070.888019
AGCAGGG32450.064.5677648
AAGCAGG50950.059.0522587
CAAAAGC48900.057.3831674
TTAGTAG1154.0017767E-1156.7449261
GCGCGAA651.0933221E-555.774931
TACTAAT1850.054.864187
GCAGGGG9200.054.374329
ACAAGGG7900.054.1448947
AGGCGTC2300.053.592436
CTACTAA1900.053.426516
ACTAATA1950.052.0506368
TAGGCGT2400.051.3594135
ACAGACT4700.050.9036258
TAAACTA3850.048.966045
TCTATGC14500.048.502183
Produced by FastQC (version 0.11.5)