FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_neg.4.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_neg.4.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62140
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT820213.199227550691987No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG10091.6237528162214354No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC10061.6189250080463469No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC5000.804634695848085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4920.7917605407145155No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT4880.7853234631477308No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT3960.6372706791116833No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGG3360.5407145156099131No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT2920.4699066623752816No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGGCGT2470.39748953974895396No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATGTCGTATGCCGT2220.35725780495654974No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATTCCGT1670.2687479884132604No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1600.2574831026713872RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGCCTCCTATCTCGTATGCCGT1270.2043772127454136No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTCTCTCGTATGCCGT1260.20276794335371742No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTTTGCCGT1180.18989378822014805No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGGATGCCGT1150.18506598004505956No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTAGTATGCCGT920.14805278403604766No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCCT900.14483424525265529No Hit
TCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGC890.14322497586095911No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCC800.1287415513356936No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCG640.10299324106855488No Hit
CTTATACACATCTCCGAGCCAACGAGACGGACTCCTATCTCGTATGCCGT640.10299324106855488No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGA100.0070595806145.105564
GCTACAG151.2209511E-4145.105563
ACCCGAT100.007076615144.988727
CTTATAC27500.082.90891
ACATCTC27400.082.8129048
CATCTCC27400.082.5483259
TATACAC27700.082.505873
TACACAT27750.081.8343055
ACACATC27700.081.72016
TTATACA27800.081.687122
CACATCT27750.081.507177
ATACACA28150.080.4137344
ATACACC501.9883538E-458.0422254
ACAGGCG400.006006960354.4145855
TACTTTC400.006026155354.370777
CCTTATA553.1713617E-452.8082161
TATACCC450.00956935748.368523
TACACCT450.00956935748.368525
TAGAGAC450.00959989348.3295759
ACAGCCT450.00959989348.3295758
Produced by FastQC (version 0.11.5)