FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_9109.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_9109.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19594236
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG130050.018.897646145
TACACCG23500.017.2787255
CTCCGAC52000.016.86943145
TATACTG215600.016.378415
TACACTG159700.015.4824965
CCACGAC78050.015.140251145
GTATAGG70000.014.0902921
CTACACT115450.014.0684294
GTATTAG129150.014.038631
GTACTAG59250.013.95391
CCTACAC86550.013.6552223
TCTATAC138300.013.5786263
TGCCGTG90250.013.495235145
CTAGTAC58750.013.4523113
CTTACAC112400.013.3531063
TACACAG194650.013.2985825
TAGACAG236550.013.27261455
GTATAAG121450.013.2566741
TAGACTG128100.013.2451925
GTGTAGG80700.013.2106111
Produced by FastQC (version 0.11.5)