FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_8404.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_8404.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18603871
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG134950.016.4409645
TATACTG187400.016.2114985
TACACCG52850.014.5425195
TATACCG36400.014.3420045
GTATAAG130850.014.0776391
CTATACT126350.013.9446434
TATACAG167950.013.6422435
CTACACT106800.013.5100894
GTATAGA129250.013.07361
GTGTAGA122750.013.0569161
GTATAGG86350.013.0178471
CCTATAC75300.012.9990873
TAGACTG106350.012.8855535
GTCCTAG67700.012.8547131
GTATTAG142700.012.806971
CTAGGAC67400.012.6939323
TAGACAG203450.012.6160765
CTAGCAC85950.012.5693983
CCTACAC96150.012.517943
GTGTAGG92750.012.5105351
Produced by FastQC (version 0.11.5)