FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_8404.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_8404.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16837617
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG64000.014.9571231
TATACTG178950.014.8704885
TACACTG138700.014.7422415
TACACCG32950.014.3037145
TATACAG155000.013.9404065
GTGTAGA108300.013.7271221
GTGTAGG79950.013.6058871
CTCCGAC42000.013.463684145
TATACCG23950.013.3210375
CTACACT105350.013.1458424
CTATACT123350.013.0497874
CTTACAC109850.013.0030173
GTATAGA116900.012.8413271
CTAGGAC68050.012.7858933
GTATTAC108200.012.7344571
CCTACAC85050.012.7025213
TACACAG171100.012.5862785
TCTATAC119200.012.4088593
TCTCCGC129150.012.405564145
GTGCTAG87950.012.28583051
Produced by FastQC (version 0.11.5)