FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_6852.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_6852.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17351200
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC89150.018.62255145
TATACTG216900.016.1458665
TACACTG155300.015.9671895
CTACACT114950.014.7597664
GTATAGG66700.014.4604231
CTCCGAC29850.014.3295555145
GTATAGA129700.013.8665021
TCTCCGC114600.013.601252145
CTATACT134050.013.5762394
GTCCTAG81250.013.5667371
ATGCCGG91050.013.536114145
GACTCCG68050.013.4232437
CCTACAC80450.013.1581743
GTATTAG129600.013.1497681
TATACAG173550.013.118345
GTCTAGG81400.013.0962851
CTTACAC115600.012.9831873
TCTACAC117600.012.9473483
TACACAG192200.012.8639645
ATACTGT276600.012.68726
Produced by FastQC (version 0.11.5)