FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_6751.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_6751.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19061280
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG174350.017.1333565
TACACTG131950.016.0450335
CTATACT142850.015.277424
ATGCCGG128850.015.022872145
CTTACAC110800.014.723233
GTATAGA142100.014.646531
CTACACT114650.014.6083664
TATACCG41100.014.112875
CCTACAC94150.014.0925993
GTATAGG84300.013.5057591
TACACAG156800.013.4559415
TATACAG175100.013.3332775
GTATAAG139950.013.2652061
GTGTAGG95900.013.1576261
GTGTAGA131850.013.0351231
GTGTAAG108050.013.0203871
GTCCTAG68800.012.9647331
TCTATAC168150.012.8061893
TAGACTG105400.012.6573965
GTATTAG165600.012.6118581
Produced by FastQC (version 0.11.5)