FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_6331.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_6331.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17223509
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG140150.016.5553385
TATACTG196750.016.2887845
CTACACT107900.014.7836324
TCTATAC123700.014.2433683
CTATACT122150.013.8900024
TACACCG31700.013.2663035
ATACTGT257200.013.2216096
TCTCCGC114150.013.210465145
TAGACAG208000.013.176795
GTATTAG126800.013.0400331
CTTACAC108950.012.9772743
CCTACAC82950.012.761823
TATACAG171350.012.7368945
GTATAAG122100.012.7104591
CTAGACT94200.012.700274
ATACCGT38700.012.5529646
TCTACAC113000.012.4479943
GTGTAGG78800.012.3322331
CTAATAC140000.012.3260813
GCTAGAC72250.012.2432893
Produced by FastQC (version 0.11.5)