FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_6119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_6119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16151757
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG139550.016.8858685
TATACTG192350.015.5301235
CTACACT99800.014.675384
GTGTAGG72550.014.5931321
CCTACAC70000.013.9828223
GTATAGA121500.013.72728351
CTATACT117950.013.6465714
TCTCCGC133150.013.448522145
TAGACTG116500.013.4430465
TTACACT157000.012.7922994
CCTATAC64450.012.7120223
ATGCCGG77950.012.648573145
GTATAAG110200.012.6343441
GTGTAAG100500.012.4828321
CTTACAC102450.012.45546153
CTCCGAC30900.012.434725145
GTCCTAA80500.012.4312931
GTATTAG120200.012.3675161
ATACTGT250700.012.3204096
CTAGGAC68250.012.2167113
Produced by FastQC (version 0.11.5)