FastQCFastQC Report
Wed 1 May 2019
HCGGGBGXB_n01_MG_4340.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGGGBGXB_n01_MG_4340.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17792668
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG38300.016.278555145
TATACTG199650.016.2343065
GTGTAGG83550.015.190561
CTCCGAC37800.015.151355145
CTATACT132850.015.0095164
GTATAGG71650.014.7781081
CCTATAC72300.014.6420373
TCTCCGC181350.014.151479145
GTATAGA134400.014.0299541
TACACAG181050.014.0173215
ATGCCGG162050.014.00269145
CCACGAC57800.013.922311145
TACACCG28000.013.7250325
TATACAG170800.013.71229655
TCTATAC132450.013.6859513
AGACGGC237800.013.627364145
TACACTG148950.013.5818585
CCTACAC85350.013.3377663
CTACACT109300.013.2679964
GTACTAG56950.013.2440811
Produced by FastQC (version 0.11.5)